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Molecular Biology (PCR)

• PCR is a DNA polymerase reaction and as with any polymerase reaction it requires a DNA template and a free 3′-OH. The template is provided by the DNA sample to be amplified and the free 3′-OH are provided by site-specific oligonucleotide primers. The primers are complementary to each of the ends of the sequence that is to be amplified. The three steps of the reaction are : Denaturation , Annealing , Primer Extension and we have all requirements for each step